ev a71 strain h vr 1432 Search Results


ev a71 strain h vr 1432  (ATCC)
99
ATCC ev a71 strain h vr 1432
Ev A71 Strain H Vr 1432, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ev+a71+strain+h+vr+1432/Human+enterovirus+71/pmc08315976-307-0-8
Average 99 stars, based on 1 article reviews
ev a71 strain h vr 1432 - by Bioz Stars, 2026-10
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ev71 strain brcr vr 1775 andh  (ATCC)
94
ATCC ev71 strain brcr vr 1775 andh
FIGURE 2 | LY-55 inhibited <t>EV71</t> and CVA16 replication in vitro. (A) Inhibition of viral titer by LY-55. (B) Effects of LY-55 on EV71 or CVA16-induced CPE in Vero cells were determined via crystal violet staining. (C,D) Vero cells (9 × 105 cells/well) were plated into 6-well culture plates and infected with EV71 or CVA16 (MOI = 0.1) for 1 h. The infected cells were treated with the indicated concentrations of LY-55 for 24 h. Intracellular viral VP1 protein (C) and RNA (D) were determined by using Western blot and qRT-PCR assays, respectively. **P < 0.01, *P < 0.05. (E) EV71 or CVA16-infected cells were revealed by using immunofluorescent detection of VP1 protein by using fluorescence microscopy (×100).
Ev71 Strain Brcr Vr 1775 Andh, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ev+a71+strain+h+vr+1432/Enterovirus+A71/pm31448243-31-0-9
Average 94 stars, based on 1 article reviews
ev71 strain brcr vr 1775 andh - by Bioz Stars, 2026-10
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staphylococcus sp  (ATCC)
94
ATCC staphylococcus sp
FIGURE 2 | LY-55 inhibited <t>EV71</t> and CVA16 replication in vitro. (A) Inhibition of viral titer by LY-55. (B) Effects of LY-55 on EV71 or CVA16-induced CPE in Vero cells were determined via crystal violet staining. (C,D) Vero cells (9 × 105 cells/well) were plated into 6-well culture plates and infected with EV71 or CVA16 (MOI = 0.1) for 1 h. The infected cells were treated with the indicated concentrations of LY-55 for 24 h. Intracellular viral VP1 protein (C) and RNA (D) were determined by using Western blot and qRT-PCR assays, respectively. **P < 0.01, *P < 0.05. (E) EV71 or CVA16-infected cells were revealed by using immunofluorescent detection of VP1 protein by using fluorescence microscopy (×100).
Staphylococcus Sp, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ev+a71+strain+h+vr+1432/Staphylococcus+sp/custom%40155%4010%2E21203%2Frs%2E3%2Ers-806112
Average 94 stars, based on 1 article reviews
staphylococcus sp - by Bioz Stars, 2026-10
94/100 stars
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FIGURE 2 | LY-55 inhibited EV71 and CVA16 replication in vitro. (A) Inhibition of viral titer by LY-55. (B) Effects of LY-55 on EV71 or CVA16-induced CPE in Vero cells were determined via crystal violet staining. (C,D) Vero cells (9 × 105 cells/well) were plated into 6-well culture plates and infected with EV71 or CVA16 (MOI = 0.1) for 1 h. The infected cells were treated with the indicated concentrations of LY-55 for 24 h. Intracellular viral VP1 protein (C) and RNA (D) were determined by using Western blot and qRT-PCR assays, respectively. **P < 0.01, *P < 0.05. (E) EV71 or CVA16-infected cells were revealed by using immunofluorescent detection of VP1 protein by using fluorescence microscopy (×100).

Journal: Frontiers in cellular and infection microbiology

Article Title: Lycorine Derivative LY-55 Inhibits EV71 and CVA16 Replication Through Downregulating Autophagy.

doi: 10.3389/fcimb.2019.00277

Figure Lengend Snippet: FIGURE 2 | LY-55 inhibited EV71 and CVA16 replication in vitro. (A) Inhibition of viral titer by LY-55. (B) Effects of LY-55 on EV71 or CVA16-induced CPE in Vero cells were determined via crystal violet staining. (C,D) Vero cells (9 × 105 cells/well) were plated into 6-well culture plates and infected with EV71 or CVA16 (MOI = 0.1) for 1 h. The infected cells were treated with the indicated concentrations of LY-55 for 24 h. Intracellular viral VP1 protein (C) and RNA (D) were determined by using Western blot and qRT-PCR assays, respectively. **P < 0.01, *P < 0.05. (E) EV71 or CVA16-infected cells were revealed by using immunofluorescent detection of VP1 protein by using fluorescence microscopy (×100).

Article Snippet: EV71 strain BrCr (VR-1775) andH (VR-1432) were purchased from ATCC.

Techniques: In Vitro, Inhibition, Staining, Infection, Western Blot, Quantitative RT-PCR, Microscopy

FIGURE 4 | LY-55 could inhibit autophagy induced by EV71 and CVA16 infection. (A,B) LY-55 and lycorine can reduce EV71 and CVA16 induced autophagy. Vero cells mock-infected or infected with EV71 or CVA16 (MOI = 0.1) for 1 h. Cells were treated with LY-55 and lycorine for 24 h. Cells were harvested, and the proteins were examined by using Western blot analysis. (C,D) LY-55 and 3-MA could synergistically inhibit virus replication through inhibiting autophagy. Vero cells mock-infected or infected with EV71 or CVA16 (MOI = 0.1) for 1 h. Cells were treated with LY-55 and 3-MA for 24 h. Cells were harvested, and the proteins were examined by using Western blot analysis. Sorfware “Gel-Pro analyzer” was used to analysis of the optical density ratio of the bands.

Journal: Frontiers in cellular and infection microbiology

Article Title: Lycorine Derivative LY-55 Inhibits EV71 and CVA16 Replication Through Downregulating Autophagy.

doi: 10.3389/fcimb.2019.00277

Figure Lengend Snippet: FIGURE 4 | LY-55 could inhibit autophagy induced by EV71 and CVA16 infection. (A,B) LY-55 and lycorine can reduce EV71 and CVA16 induced autophagy. Vero cells mock-infected or infected with EV71 or CVA16 (MOI = 0.1) for 1 h. Cells were treated with LY-55 and lycorine for 24 h. Cells were harvested, and the proteins were examined by using Western blot analysis. (C,D) LY-55 and 3-MA could synergistically inhibit virus replication through inhibiting autophagy. Vero cells mock-infected or infected with EV71 or CVA16 (MOI = 0.1) for 1 h. Cells were treated with LY-55 and 3-MA for 24 h. Cells were harvested, and the proteins were examined by using Western blot analysis. Sorfware “Gel-Pro analyzer” was used to analysis of the optical density ratio of the bands.

Article Snippet: EV71 strain BrCr (VR-1775) andH (VR-1432) were purchased from ATCC.

Techniques: Infection, Western Blot, Virus

FIGURE 5 | LY-55 treatment reduced the mortality of mice upon lethal EV71 challenge and reduced the EV71 replication post sub-lethal dose EV71 challenge in vivo. (A) 12-day ICR mice were infected with 10 LD50 of EV71-mouse adapted strain (EV71-H-MA), followed by administration of LY-55 and lycorine once a day at the indicated doses. Mice survival was observed for 14 days. (B–E) 12-day ICR mice were infected with 1 LD50 of EV71-mouse adapted strain (EV71-H-MA), followed by LY-55 and lycorine administration once a day at the indicated doses. Eight mice enrolled in each group were intraperitoneally injected with LY-55 or lycorine for 7 days consecutively and weighed daily for 12 days. (B) Eight mice enrolled in each group were dissected at 5 dpi. Muscle tissues prepared from the four mice were used for viral protein expression and viral titer assays. Muscle tissues prepared from another four mice were applied in pathological and IHC analyses. The expression levels of VP1 protein in the muscles were determined by using Western blot analysis at 5 dpi (n = 4) (C). Vero cells were infected with a challenge of the diluted muscle homogenates at 5 dpi, and the viral titers were determined by –Log10TCID50 (n = 4) (D). The muscle tissue sections prepared from mice at 5 dpi were stained with EV71 VP1 antibody (E) and H&E (F). Each value represents mean ± SD. *P < 0.05, **P < 0.01, vs. EV71-infected mice treated with water.

Journal: Frontiers in cellular and infection microbiology

Article Title: Lycorine Derivative LY-55 Inhibits EV71 and CVA16 Replication Through Downregulating Autophagy.

doi: 10.3389/fcimb.2019.00277

Figure Lengend Snippet: FIGURE 5 | LY-55 treatment reduced the mortality of mice upon lethal EV71 challenge and reduced the EV71 replication post sub-lethal dose EV71 challenge in vivo. (A) 12-day ICR mice were infected with 10 LD50 of EV71-mouse adapted strain (EV71-H-MA), followed by administration of LY-55 and lycorine once a day at the indicated doses. Mice survival was observed for 14 days. (B–E) 12-day ICR mice were infected with 1 LD50 of EV71-mouse adapted strain (EV71-H-MA), followed by LY-55 and lycorine administration once a day at the indicated doses. Eight mice enrolled in each group were intraperitoneally injected with LY-55 or lycorine for 7 days consecutively and weighed daily for 12 days. (B) Eight mice enrolled in each group were dissected at 5 dpi. Muscle tissues prepared from the four mice were used for viral protein expression and viral titer assays. Muscle tissues prepared from another four mice were applied in pathological and IHC analyses. The expression levels of VP1 protein in the muscles were determined by using Western blot analysis at 5 dpi (n = 4) (C). Vero cells were infected with a challenge of the diluted muscle homogenates at 5 dpi, and the viral titers were determined by –Log10TCID50 (n = 4) (D). The muscle tissue sections prepared from mice at 5 dpi were stained with EV71 VP1 antibody (E) and H&E (F). Each value represents mean ± SD. *P < 0.05, **P < 0.01, vs. EV71-infected mice treated with water.

Article Snippet: EV71 strain BrCr (VR-1775) andH (VR-1432) were purchased from ATCC.

Techniques: In Vivo, Infection, Injection, Expressing, Muscles, Western Blot, Staining

FIGURE 6 | LY-55 treatment protected mice from apparent symptoms post sub-lethal dose EV71 challenge. 12-day ICR mice were infected with 1 LD50 of EV71-mouse adapted strain (EV71-H-MA), followed by daily administration of LY-55 and lycorine at the indicated doses. Eight mice enrolled in each group were intraperitoneally injected with LY-55 or lycorine for 7 consecutive days and observed for morbidity daily for 12 days.

Journal: Frontiers in cellular and infection microbiology

Article Title: Lycorine Derivative LY-55 Inhibits EV71 and CVA16 Replication Through Downregulating Autophagy.

doi: 10.3389/fcimb.2019.00277

Figure Lengend Snippet: FIGURE 6 | LY-55 treatment protected mice from apparent symptoms post sub-lethal dose EV71 challenge. 12-day ICR mice were infected with 1 LD50 of EV71-mouse adapted strain (EV71-H-MA), followed by daily administration of LY-55 and lycorine at the indicated doses. Eight mice enrolled in each group were intraperitoneally injected with LY-55 or lycorine for 7 consecutive days and observed for morbidity daily for 12 days.

Article Snippet: EV71 strain BrCr (VR-1775) andH (VR-1432) were purchased from ATCC.

Techniques: Infection, Injection